FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686010
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1618461.8632951147880328No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT305420.35162289704939326No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130880.15067908049841067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT280500.0101.363971
AGCAGTG299600.094.877872
GTATCAA302250.094.233899
GGTATCA312600.091.182958
TGGTATC320600.088.4360357
GTGGTAT324250.087.5510566
AGTGGTA328250.086.308215
CAGTGGT329750.086.199444
GCAGTGG411800.069.356633
AGAGTAC307150.025.00774415-19
AACGCAG321650.024.56731210-14
CAACGCA313600.024.41739710-14
TCAACGC303800.024.3708910-14
GTACTTT311200.022.71203420-24
CAGAGTA352650.021.20550715-19
ATCAACG298650.020.7653610-14
GCAGAGT352050.020.66091715-19
TACTTTT378350.019.4079220-24
CTACACG7600.019.2099131
TATCAAC328200.018.4929478
Produced by FastQC (version 0.11.5)