FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12166029
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT44400.098.3545761
CGATTGA40900.095.185031
AACGTTG61200.092.559351
ATGCGAT30350.090.319161
CCGTACT64700.089.918561
GATCGTA35700.085.156641
ACGGGTC48650.083.318731
TACTCGC63400.083.138021
TACCTTA57900.080.45871
ACGATAC36100.078.962391
ATCCGAA58600.077.2582241
CGAACAT46400.077.146151
CGATAGT28450.076.917944
CGTAACA26300.076.910713
TACGGGC50150.076.901611
TACACGA44600.076.826971
CCGGTAG49550.076.361491
CTCTACG51000.076.334661
ATAGACC55650.076.1134951
AGACTAG49450.074.6844568
Produced by FastQC (version 0.11.5)