FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10924865
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA38150.098.746191
TACGGAT38650.095.206411
CCGTACT58750.092.276131
ATGCGAT25700.090.727971
AACGTTG51750.089.973551
GATCGTA32750.085.2141651
TACACGA41400.082.722161
CGTAACA25150.081.5732653
TCGTTAC35500.080.7126168
ACGGGTC45650.080.128551
CGCTATC46300.079.94791
CGAACAT41800.079.664391
TACTCGC56750.078.9648061
CGTCTAG25050.078.7461857
TACGGGC48650.077.1345141
TACCTTA55650.076.4666441
ACGATAC31800.076.302981
CGTTCTG82750.075.903821
ATAGACC50000.075.197621
CGATCGG46750.075.1259161
Produced by FastQC (version 0.11.5)