FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10915168
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA36150.097.41551
TACGGAT39450.094.984161
CCGTACT61500.092.6355441
AACGTTG51900.091.545461
ATGCGAT24700.086.310111
ACGATAC32950.085.8984151
GATCGTA32250.083.4762651
TACTCGC52250.082.71631
CGAACAT39350.082.467181
CGTCTAG25850.082.4361657
TACGGGC49750.081.315381
CGTAACA23500.079.6475143
TACACGA39300.079.609871
GCGCGAT47400.077.978771
CGCTATC47100.077.703061
ACGGGTC46050.077.420761
GACGTTA41550.077.400231
CGAATGT44200.076.545851
TCGTTAC32250.076.345438
TACCTTA52450.076.158411
Produced by FastQC (version 0.11.5)