FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9061653
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1597041.7624157535054585No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT300910.3320696566068023No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110560.12200864455966258No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91210.10065492465888949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT270050.095.504121
AGCAGTG276050.093.500432
GTATCAA277600.092.4596569
GGTATCA283150.090.672788
AGTGGTA290900.088.403685
CAGTGGT293400.087.724024
TGGTATC295850.086.853477
GTGGTAT298300.086.091866
GCAGTGG367050.070.612223
AGAGTAC280700.024.399315-19
TCAACGC274200.023.5408910-14
GTACTTT286700.021.88135720-24
CAACGCA307750.021.82605210-14
CTACACG11050.021.7947251
AACGCAG322850.021.5901610-14
ATCAACG270750.020.22457710-14
GCAGAGT326150.019.78522915-19
TACTTTT352150.018.7835520-24
CAGAGTA359300.018.62095815-19
ACGCAGA290550.017.66547215-19
Produced by FastQC (version 0.11.5)