FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9201183
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1572891.7094432313757915No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298580.32450175156824945No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112350.12210386425310746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT276950.096.657851
GTATCAA285250.093.829
AGCAGTG286050.093.7613142
GGTATCA293750.091.01938
CAGTGGT298950.089.367734
AGTGGTA299050.089.3422245
GTGGTAT303400.088.178976
TGGTATC306200.087.36557
GCAGTGG381000.070.915633
AGAGTAC290950.024.4772515-19
TCAACGC283750.023.73736410-14
CAACGCA317050.022.10724310-14
GTACTTT297100.022.01821320-24
AACGCAG331050.021.8335610-14
ATCAACG279900.020.42110310-14
GCAGAGT332600.020.26484315-19
TACTTTT358900.019.07739320-24
CAGAGTA372600.018.67286515-19
ACGCAGA302250.017.75973315-19
TATCAAC313450.017.2337510-14
Produced by FastQC (version 0.11.5)