FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10186242
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA36150.096.425321
CCGTACT53450.092.502521
TACGGAT34700.092.258541
AACGTTG51250.090.497131
GATCGTA28350.089.51541
ATGCGAT25900.087.0022051
CGAACAT40450.082.208711
ACGGGTC43300.082.018651
TACGGGC46050.080.762941
AGACTAG39350.079.400488
TACTCGC50300.078.868291
CGTAACA21250.078.284193
ACGATAC28400.076.519031
GACGTTA41050.076.0331
ATAGACC44150.075.979891
CGATCGG43450.075.693451
TACACGA36650.075.610341
TCGTTAC30850.075.608048
CGTCTAG23900.075.60567
TACCTTA47200.074.778181
Produced by FastQC (version 0.11.5)