FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10356147
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1752721.6924441107295987No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331870.3204570193914783No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124060.11979358732547925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT296950.093.651821
GTATCAA308600.089.8270959
AGCAGTG311750.089.34782
GGTATCA316600.087.625938
AGTGGTA321950.086.506865
CAGTGGT326150.085.083994
TGGTATC331300.083.954787
GTGGTAT331600.083.835826
GCAGTGG412550.067.843183
AGAGTAC312000.024.15594715-19
TCAACGC303950.023.42940310-14
GTACTTT320150.021.64854220-24
CAACGCA342450.021.63624410-14
AACGCAG360000.021.36538110-14
ATCAACG301500.019.76593610-14
GCAGAGT363000.019.572115-19
TACTTTT394350.018.60130520-24
CAGAGTA401200.018.36894615-19
TATCAAC337750.017.1566478
ACGCAGA326150.016.95347615-19
Produced by FastQC (version 0.11.5)