FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825006
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2989032.527719647668678No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT671710.5680419950738291No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT253300.214207079472095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144730.12239317257006044No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130240.11013947899899586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT460300.0109.486411
AGCAGTG488650.0103.2861562
GTATCAA555900.090.957949
CAGTGGT563200.089.495844
GGTATCA567800.089.1023568
TGGTATC575050.087.503237
AGTGGTA582800.086.436625
GTGGTAT593650.084.761266
GCAGTGG639050.079.267733
CTACACG10600.026.8532141
AGAGTAC552900.025.66492315-19
AACGCAG596650.024.42722110-14
CAACGCA580400.024.37928410-14
TCAACGC561300.024.22123510-14
TACACGA12300.023.4140662
GTACTTT559550.023.40310920-24
ATCAACG553500.022.7632929
GCAGAGT595650.022.42344315-19
CAGAGTA621600.022.3026315-19
TATCAAC606800.020.8350038
Produced by FastQC (version 0.11.5)