FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11957254
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA147630.12346480220291381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC36050.0101.106751
GATCGTA34150.097.571381
ATGCGAT32950.093.3969351
TAAACCG42250.092.98541
CGCTATC44500.088.283891
CGTTAGA51850.087.415211
ATTCGCG23300.086.24092
CGATTGA30850.085.369171
GCGCAAC48150.083.404691
CATTCGC66700.082.132841
GATCTAG47050.082.107461
TCGTTAC37900.082.0600058
GTTAAGC53200.082.051651
TCGTAGA47850.081.1908341
CGAATGT42850.080.477641
ACGGGCT104350.080.3867651
TACTTAC74250.078.190531
TATGCCC48450.078.083141
ATCGAGT49150.076.8230441
GAACCTA54000.076.390081
Produced by FastQC (version 0.11.5)