FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11957254
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2968302.482426149013812No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT662400.5539733453851529No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT248620.20792399325129332No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139320.11651504601307291No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT127980.10703126319805534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT465450.0109.592731
AGCAGTG489550.0104.282262
GTATCAA549550.092.367389
CAGTGGT557000.091.4775544
GGTATCA562450.090.651758
TGGTATC574500.088.47657
AGTGGTA584150.087.262125
GTGGTAT592700.085.8406756
GCAGTGG641400.079.732143
CTACACG10550.028.4293211
AGAGTAC549650.025.69195415-19
AACGCAG592600.024.43195310-14
CAACGCA574150.024.23907310-14
TCAACGC556950.024.20951810-14
TACACGA13050.023.7659362
GTACTTT557500.023.27500320-24
GCAGAGT593450.022.43723115-19
CAGAGTA616200.022.16957715-19
ATCAACG549750.021.999829
TACTTTT662100.020.38519320-24
Produced by FastQC (version 0.11.5)