FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8720122
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2126462.438566799868167No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473150.5425956196484407No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180080.20651087221027412No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100730.11551443890349242No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT89160.1022462759121948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT337800.0109.461841
AGCAGTG360150.0102.738322
GTATCAA400400.091.999169
GGTATCA409450.090.003478
CAGTGGT411100.089.72674
TGGTATC416350.088.2342157
AGTGGTA425850.086.7735
GTGGTAT431850.085.300686
GCAGTGG471100.078.7879643
CTACACG8450.028.5609441
AGAGTAC401200.025.64134615-19
AACGCAG435500.024.36258710-14
CAACGCA423500.024.24716610-14
TCAACGC409000.024.22318610-14
GTACTTT407550.023.31506520-24
TACACGA10600.023.0945932
ATCAACG403550.022.9763539
GCAGAGT432050.022.44414515-19
CAGAGTA451450.022.17492315-19
ACGACGC11050.022.1506585
Produced by FastQC (version 0.11.5)