FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8736141
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2178662.4938471116709313No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT488940.5596750327175352No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT185480.21231342305487055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.1169395045249384No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT92930.10637419885965668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT340550.0108.985771
AGCAGTG360250.0103.30642
GTATCAA407900.091.733119
CAGTGGT408100.091.174964
GGTATCA417400.089.662528
TGGTATC422250.087.933557
AGTGGTA424700.087.59435
GTGGTAT436950.085.007736
GCAGTGG465950.080.256883
CTACACG8700.035.2431871
TACACGA9850.030.6999512
AGAGTAC406200.025.73266215-19
AACGCAG437400.024.4494110-14
TCAACGC411600.024.32025710-14
CAACGCA425700.024.31297910-14
ACGACGC12950.023.3506625
GTACTTT413250.023.29080620-24
GCAGAGT435200.022.54907215-19
ATCAACG406850.022.2270819
CAGAGTA456950.022.165815-19
Produced by FastQC (version 0.11.5)