FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7743854
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1970412.544482372730684No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT434670.5613096527904581No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT166710.21528040172244983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94070.12147698032530056No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT83770.10817610972520919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT305450.0110.572871
AGCAGTG323950.0104.258572
GTATCAA367200.091.846789
CAGTGGT366950.091.5972444
GGTATCA374550.090.106158
TGGTATC380850.088.160817
AGTGGTA385650.087.435175
GTGGTAT393400.085.6228566
GCAGTGG422000.080.126013
AGAGTAC365850.025.67184615-19
CTACACG6400.025.1298241
AACGCAG395250.024.38138610-14
CAACGCA385350.024.19323210-14
TCAACGC371850.024.09193410-14
GTACTTT372550.023.1494620-24
GCAGAGT396900.022.23056415-19
CAGAGTA413150.022.15425915-19
ATCAACG365750.021.4338689
TACTTTT441450.020.40733720-24
TATCAAC401200.019.4511388
Produced by FastQC (version 0.11.5)