FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7848139
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA97230.12388924304220401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC22400.098.929671
ATGCGAT20600.098.019731
GATCGTA24550.091.453471
TAAACCG28150.090.6338961
CGTTAGA34700.089.701431
CGATTGA21600.088.757071
ATTCGCG14700.088.1602862
GATCTAG31150.084.713271
GTTAAGC35550.083.455651
TACTTAC47750.082.4368441
CATTCGC43250.082.418321
ATCGAGT31700.081.863761
CGCTATC28600.081.3065641
TAGTGGT37800.080.223661
ACGGGCT67350.079.6601641
TCGTTAC24850.079.652038
CCGGGAT52550.078.097421
GCGCAAC32100.077.664411
TACGGGC33100.077.520341
CGATGTA33150.077.183521
Produced by FastQC (version 0.11.5)