FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8659849
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT24400.096.910541
ACGATAC28400.094.021071
CGATTGA23750.087.921641
ATCGAGT38950.085.179461
CTAATGG28100.084.1500851
CCGTACT36550.083.2082441
ACGGGTC47050.082.886281
TAAACCG22600.081.771651
TACCTTA42200.081.5504151
GATCGTA20550.081.43171
ACGTCAA36050.080.527671
TACACGA30100.080.250751
TTAGGCA30450.080.045141
CGTAACA19250.079.283793
TCGTAGA27200.078.909741
GCGCAAC32700.078.542451
TACGGAT26150.078.461191
CTGATAG31700.078.265911
GTAACGA18250.078.1104668
CAACTAG27700.078.01071
Produced by FastQC (version 0.11.5)