FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9029458
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC30050.0100.638611
ATGCGAT23000.095.050511
CGATTGA23300.089.760851
GTAACGA19500.084.9163368
ATCGAGT37350.084.871061
TACCTTA41700.084.0562441
CCGTACT36650.083.708421
TACGGAT28650.082.410281
GTTAAGC36200.081.930731
TGCGATA19000.079.572639
CTAATGG29450.078.6869661
AGCGATA22250.077.980328
TAACGAC21250.077.9232259
CTGATAG35000.077.4525761
TAAACCG23450.076.756491
ACGGGTC52400.076.487841
CGCTATC29450.076.459981
TCGTTAC24500.076.40218
CGAATGT31600.076.100561
ACGATGT34650.075.711231
Produced by FastQC (version 0.11.5)