FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9029458
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1618101.792023397196155No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT335740.3718274120107763No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133450.1477940314911482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG286000.099.0513462
AAGCAGT291700.096.935651
GTATCAA311350.091.090569
GGTATCA320150.088.4073648
CAGTGGT327200.086.813994
TGGTATC333750.084.674977
GTGGTAT335550.084.220746
AGTGGTA339750.083.5853965
GCAGTGG395400.072.442953
CTACACG8300.026.3817461
AGAGTAC305200.025.43884715-19
TCAACGC314800.023.91097510-14
CAACGCA329600.023.66727410-14
AACGCAG343150.023.53831110-14
TACACGA9400.022.9818742
GTACTTT318050.022.55161920-24
GCAGAGT337350.021.64010215-19
ATCAACG308800.021.492149
CAGAGTA372100.020.26538815-19
TATCAAC344250.019.279048
Produced by FastQC (version 0.11.5)