FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8950880
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1565151.7485990204315107No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328920.36747224853869115No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130290.14556110684089163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT283600.098.035991
AGCAGTG286500.097.048272
GTATCAA306600.090.799729
GGTATCA317450.088.019228
CAGTGGT325050.085.429654
TGGTATC330400.083.959657
AGTGGTA334050.083.408195
GTGGTAT333600.083.267946
GCAGTGG390800.071.7197953
CTACACG8600.029.7767831
AGAGTAC305600.025.33986915-19
TCAACGC310800.024.07044410-14
CAACGCA326350.023.74853110-14
AACGCAG339900.023.47954610-14
TACACGA10900.023.1655312
GTACTTT315600.022.59212720-24
ATCAACG307100.022.5750629
GCAGAGT334500.021.88092215-19
ACGACGC12200.020.6546295
CAGAGTA370700.020.38497515-19
Produced by FastQC (version 0.11.5)