FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10617116
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT29100.099.1933441
ACGATAC32450.095.0027541
ATCGAGT46100.089.7421341
CGATTGA28250.086.478041
TGCGATA24850.084.592749
CCGTACT46150.083.0278851
TACCTTA49150.082.693871
GATCGTA27800.082.3855061
CAACTAG36450.082.382991
CGTAACA24150.082.270483
GCGCAAC42450.081.1868441
TAAACCG29650.079.206871
CGCTATC38150.079.09311
GTAACGA22700.078.650688
TTAGGCA36900.078.6197361
CTCTACG35300.078.475721
TCGTAGG30200.078.4278
CTGATAG40550.078.3566061
GACGTTA42750.078.236021
GCATGCG40350.077.4836351
Produced by FastQC (version 0.11.5)