FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11205094
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT29450.097.066731
ACGATAC34200.095.587231
CGATTGA28300.091.169021
CCGTACT47050.085.994471
TACCTTA51300.084.728281
ATCGAGT48000.084.2924961
TAAACCG29800.083.136471
TGCGATA25000.081.773039
CAACTAG39000.080.8155441
TACACGA39050.080.7120741
ACGATGT41500.080.53931
TACGGAT33900.079.784361
CGCTATC41700.079.6256941
GCGCAAC47050.079.4514161
TTAGGCA38750.079.445391
CTGATAG43450.078.78041
CATATTC52200.078.77411
TTTATGC46000.078.715491
CTAATGG34800.078.563481
TCGTAGG30250.078.053418
Produced by FastQC (version 0.11.5)