FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12229091
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT60450.0107.0611651
CGATTGA44050.097.946981
TAAACCG52400.097.082161
ATGCGAT32950.095.774031
ACGATAC38000.094.553721
CAACTAG56600.088.075471
ATAGACC60850.087.433441
CCGTACT72850.086.5371
ATCCGAA55850.086.5178451
AGCGATA30800.085.318248
AGGCTAG80900.079.202928
AGACTAG52700.079.09848
ATTCGAC34350.078.387099
CACGCGA26900.078.150248
TATCAGG65000.077.814661
CTAATGG49250.076.654711
GATCGTA34000.075.239091
ATTCGCG23000.075.151812
GTACCGT69150.074.961818
ATCGAGT60850.074.85741
Produced by FastQC (version 0.11.5)