FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12229091
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2811022.2986336433345698No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT513420.41983496565689143No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218030.1782879855910795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT453950.0106.618971
AGCAGTG484750.099.682
GTATCAA508100.095.355439
GGTATCA519400.093.213138
AGTGGTA529100.091.5367055
TGGTATC528550.091.422047
GTGGTAT533400.090.645516
CAGTGGT544050.088.728474
GCAGTGG659450.073.836013
AGAGTAC508800.025.24242215-19
AACGCAG523850.025.1210810-14
CAACGCA515250.024.84737410-14
TCAACGC503150.024.6608510-14
GTACTTT518450.023.03705420-24
CAGAGTA576750.021.7741215-19
GCAGAGT568400.021.48850315-19
ATCAACG492350.021.3095310-14
TACTTTT626650.019.86137220-24
CGCAGAG554950.018.86437815-19
ACGCAGA517450.018.73430615-19
Produced by FastQC (version 0.11.5)