FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11018401
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2557092.320745088148453No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT460380.4178283219134973No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT202060.18338414076597864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT409000.0107.957611
AGCAGTG436750.0101.105892
GTATCAA459400.096.352549
GGTATCA468850.094.343138
TGGTATC477450.092.071217
AGTGGTA481350.091.8026055
GTGGTAT484550.090.989566
CAGTGGT490100.090.075064
GCAGTGG598050.074.1893843
AGAGTAC462450.025.4384915-19
AACGCAG476350.025.1706310-14
CAACGCA465100.025.01168410-14
TCAACGC453500.024.87038610-14
GTACTTT474550.023.05738820-24
CAGAGTA521350.022.03703315-19
GCAGAGT514250.021.69448515-19
ATCAACG445950.021.44590810-14
TACTTTT572600.019.80578220-24
CGCAGAG504150.018.95311715-19
ACGCAGA472450.018.69180315-19
Produced by FastQC (version 0.11.5)