FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10971469
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2566602.339340338107869No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT462500.4215479258064713No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT205710.1874954028489713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT405600.0106.8384861
AGCAGTG432400.0100.3540652
GTATCAA456850.094.945259
GGTATCA470350.092.2809148
TGGTATC474100.091.0799267
AGTGGTA478000.090.359695
GTGGTAT479900.089.956946
CAGTGGT487750.088.5960854
GCAGTGG589500.073.951253
AGAGTAC462150.025.36706215-19
AACGCAG475500.025.20949410-14
CAACGCA468050.024.80791910-14
TCAACGC457050.024.5954210-14
GTACTTT475250.022.83450520-24
CAGAGTA522250.021.90201815-19
GCAGAGT511500.021.68399815-19
ATCAACG447150.021.20863210-14
TACTTTT572100.019.70639620-24
CGCAGAG504850.018.76714715-19
ACGCAGA471150.018.45026615-19
Produced by FastQC (version 0.11.5)