FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11014322
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2533162.2998782857446876No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT462310.4197353227915436No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT204320.18550392843063784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT402050.0106.343741
AGCAGTG432000.099.053192
GTATCAA454450.094.013089
GGTATCA465350.091.736618
TGGTATC471100.090.480637
AGTGGTA474550.089.780985
GTGGTAT482550.088.4266
CAGTGGT485800.087.56614
GCAGTGG587500.073.0450443
AGAGTAC457300.025.28628515-19
AACGCAG472850.025.00649310-14
CAACGCA462400.024.83353610-14
TCAACGC453950.024.51858510-14
GTACTTT470250.022.88284720-24
CAGAGTA520650.021.69520215-19
GCAGAGT510850.021.42649315-19
ATCAACG445150.021.13746510-14
TACTTTT566600.019.72608420-24
CGCAGAG499100.018.7719715-19
ACGCAGA467600.018.32141715-19
Produced by FastQC (version 0.11.5)