FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8673911
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT41350.0107.595831
ATGCGAT24300.0101.649651
CGATTGA29150.0100.8296051
TAAACCG35400.097.935491
ACGATAC25200.094.238141
ATCCGAA40150.089.2700041
AGCGATA20650.085.7547458
ATAGACC43650.084.966651
CAACTAG39800.084.7143551
CCGTACT51700.084.071041
CACGCGA19300.082.801618
AGACTAG39150.082.189988
ATTCGAC24200.078.822669
TATCAGG44550.078.643431
GCGCAAC32150.077.2860261
ACGCGAA20800.077.172419
CGCTATC35450.076.914631
ATTCGCG16900.076.732092
GTACCGT48150.076.395498
TTCGCGA22950.076.215659
Produced by FastQC (version 0.11.5)