FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8889040
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT38650.0108.3372041
CGATTGA30150.0101.684331
TAAACCG39700.097.400971
ATGCGAT21900.095.100141
ACGATAC27400.088.767741
ATCCGAA38000.088.727181
AGCGATA19950.086.605828
ATAGACC46700.085.7640151
CAACTAG40900.084.394521
TATCAGG47750.082.200541
CCGTACT52800.082.0620351
AGACTAG37650.081.264848
CACGCGA20400.080.813538
ATTCGAC23300.079.406499
CGAACAA19500.079.368994
TCGTAGG21650.078.475278
CATGCGA18750.077.976132
AGGCTAG60200.077.850828
GATCGTA26350.077.6577761
CTAATGG37400.077.299771
Produced by FastQC (version 0.11.5)