FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8889040
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2084762.345315129642796No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT374640.4214628351318027No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT164570.18513810265225492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT321450.0107.0418241
AGCAGTG344400.099.9071352
GTATCAA361550.095.4464659
GGTATCA373300.092.404678
TGGTATC376900.091.4451457
AGTGGTA382300.090.420655
GTGGTAT382550.090.133216
CAGTGGT390450.088.059264
GCAGTGG468750.073.615123
AGAGTAC364400.025.147615-19
AACGCAG375100.025.01135410-14
CAACGCA369350.024.77311710-14
TCAACGC361650.024.31322910-14
GTACTTT375200.022.68257720-24
CAGAGTA413350.021.69230515-19
ATCAACG352100.021.16973110-14
GCAGAGT410200.021.13926315-19
TACTTTT452300.019.68522520-24
CGCAGAG397700.018.69714715-19
ACGCAGA371900.018.59262515-19
Produced by FastQC (version 0.11.5)