FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13420512
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA194810.14515839634136166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA47150.0103.858871
ATGCGAT37950.0101.799981
ACGATAC54050.097.2461241
ATAGACC57100.096.160021
TTACGCA31100.087.115863
TAGTACG30900.086.8929757
TACGGTA77650.086.194231
CTTACGC32200.085.930072
CAATCGA27850.085.556248
CGAACAT55500.084.799031
AGGTCCG111250.084.740261
TCGGTAA50550.083.531481
CCGGTAG59400.082.316741
TATCAGG53000.082.1598051
ACGGATA32750.081.764729
CGTTAGA41800.080.3226551
GCGCAAC43450.080.140621
CTGATAG50950.079.134771
GTTAAGC59400.078.614341
TCGTAGG39650.078.612868
Produced by FastQC (version 0.11.5)