FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13444378
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3670282.7299738225152548No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT523890.38967217375173474No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261620.19459435014397838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT523650.0114.4476851
AGCAGTG563300.0106.14982
GGTATCA595900.0100.330578
GTATCAA597800.0100.047829
TGGTATC605150.098.499537
CAGTGGT609000.097.992874
GTGGTAT615050.096.8895656
AGTGGTA633050.094.3724065
GCAGTGG713650.083.9762343
AGAGTAC592150.025.9189615-19
CAACGCA597450.025.41646410-14
TCAACGC585900.025.19989610-14
AACGCAG620550.025.12467210-14
GTACTTT602500.023.80934720-24
GCAGAGT640850.022.76082815-19
CAGAGTA665250.022.54498115-19
ATCAACG578500.022.2417410-14
CTACACG11300.021.3177091
TACTTTT708200.020.99377820-24
CGCAGAG648650.019.76585615-19
Produced by FastQC (version 0.11.5)