FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13713400
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA199250.14529584202313067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA51150.0104.598441
ATGCGAT38700.0100.9551851
ACGATAC57050.097.869631
ATAGACC61700.094.746671
TACGGTA84350.089.612481
TCGGTAA50250.089.5001751
TTACGCA28150.086.176973
AGGTCCG110100.085.2051851
ACGGATA32550.082.719499
CGAACAT50600.081.822481
TCGTAGG39950.080.912728
CCGGTAG62950.080.475541
TATCAGG62700.080.33141
ACATACG40600.079.893011
CAACTAG47200.079.531661
TACGGGC63950.079.4450761
TAGTACG32200.079.370617
CTCTACG51400.078.847241
CTGATAG52450.078.519541
GTACGTA44950.078.3235241
Produced by FastQC (version 0.11.5)