FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13713400
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3678542.682441991045255No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT516090.3763399302871644No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257420.18771420654250587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT538750.0113.975611
AGCAGTG580400.0105.960312
GGTATCA616450.099.935378
GTATCAA618100.099.6569449
TGGTATC626600.098.316217
CAGTGGT633050.097.08844
GTGGTAT635950.096.860466
AGTGGTA654650.094.392955
GCAGTGG736800.083.7922443
AGAGTAC609150.025.80855215-19
CAACGCA618550.025.36111810-14
AACGCAG642050.025.02714510-14
TCAACGC607050.024.99480410-14
GTACTTT620650.023.77977620-24
GCAGAGT656650.022.85179115-19
CAGAGTA685650.022.54679515-19
ATCAACG597600.022.20003110-14
TACTTTT731900.020.9935420-24
CGCAGAG672350.019.66467315-19
ACGCAGA629250.019.57679615-19
Produced by FastQC (version 0.11.5)