FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134107
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA187680.14289513554290367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA48900.0106.288791
ATGCGAT38000.0102.152241
ATAGACC56300.098.6450351
ACGATAC51650.098.37781
TACGGTA75600.090.9619141
TAGTACG30300.088.620667
CGAACAT50550.086.282031
TTACGCA27550.084.924013
CCGGTAG60250.084.214841
GATCGTA41850.083.722431
CGTTAGA42400.083.150751
AGGTCCG105650.082.634941
ACGGATA30900.081.777219
CTTACGC28950.081.3293462
CAACTAG45850.080.857651
GATCGAT68150.079.892541
CTCTACG48750.079.6263661
GTTAAGC58750.079.559731
CTAATGG43450.079.3010251
TCGCTAG26400.078.809318
Produced by FastQC (version 0.11.5)