FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134107
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3422782.606024147663789No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT491710.37437642315537706No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT241590.18394094094101715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT510200.0114.150511
AGCAGTG547050.0106.348882
GGTATCA580000.0100.4230658
GTATCAA583200.0100.021549
TGGTATC589000.098.940497
GTGGTAT600700.097.0781256
CAGTGGT598250.097.029324
AGTGGTA620150.094.000755
GCAGTGG703250.083.1052253
AGAGTAC571700.026.00414315-19
CAACGCA583100.025.36632310-14
TCAACGC572200.025.08695810-14
AACGCAG606800.025.06144710-14
GTACTTT580950.023.96002420-24
GCAGAGT621250.022.7318515-19
CAGAGTA646100.022.54616515-19
ATCAACG562400.022.27213910-14
TACTTTT691150.020.83765220-24
ACGCAGA589550.019.76802415-19
CGCAGAG635450.019.71326415-19
Produced by FastQC (version 0.11.5)