FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11337081
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3075552.7128235213279326No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT439480.3876482844217131No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT216430.19090451942612036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT454350.0114.2747961
AGCAGTG485650.0106.931582
GGTATCA509400.0101.897838
GTATCAA513150.0101.195269
TGGTATC519000.099.623787
GTGGTAT527450.098.101626
CAGTGGT528900.097.873084
AGTGGTA543450.095.306085
GCAGTGG611250.085.1936343
AGAGTAC506150.026.14604215-19
CAACGCA514200.025.54614410-14
TCAACGC501650.025.40462110-14
AACGCAG534800.025.24321410-14
GTACTTT515000.024.03656620-24
GCAGAGT544900.023.1162215-19
CAGAGTA569200.022.74651715-19
ATCAACG494100.022.59930610-14
TACTTTT603600.021.27656620-24
CGCAGAG555350.020.10176715-19
ACGCAGA522850.019.91666215-19
Produced by FastQC (version 0.11.5)