FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11325948
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA165430.14606282847140037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA40950.0102.339341
ATGCGAT33200.0101.903451
ACGATAC47050.097.421531
ATAGACC47400.093.018291
ACGGATA24950.087.719719
CCGGTAG52200.085.719311
TCGTAGG32950.083.027578
CGAACAT44500.082.566791
TCGGTAA42250.082.4864961
TACGGTA63050.082.046211
AGGTCCG89400.081.464911
TAGTACG27500.080.632887
CTCTACG43500.080.450711
TTACGCA23950.080.256593
CGTTAGA37050.079.3354341
TATCAGG48750.078.950541
GATCGTA35750.078.5570761
CAATCGA23100.078.538528
TCGATTG28000.077.907419
CTAATGG38450.077.771331
Produced by FastQC (version 0.11.5)