FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10413371
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA150190.14422803144149957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA39400.0104.1785051
ACGATAC39200.0100.970391
ATGCGAT28300.098.938031
ATAGACC46700.092.916141
CGAACAT39300.089.150061
TAGTACG22950.086.56977
AGGTCCG87700.085.9171451
TACGGTA57500.085.9168551
ACATACG28300.085.729031
TCGGTAA39800.085.635961
TTACGCA23050.083.455183
CCGGTAG47050.082.566251
CGTTAGA33250.082.224921
GCGCAAC39400.081.4824751
ACGGATA23800.081.3579949
CAATCGA20700.080.679158
CTGATAG40050.080.526061
GTACGTA39150.080.31781
CAACTAG35150.080.282651
GATCGTA31800.079.520351
Produced by FastQC (version 0.11.5)