FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10600406
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA151190.1426266126033286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA37200.0106.216571
ATGCGAT30600.0100.775481
ACGATAC41450.097.788141
ATAGACC45750.093.21821
TTACGCA21650.087.4451753
CAACTAG36100.086.431591
TACGGTA63650.086.130171
ACGGATA26150.085.0666969
CGAACAT38600.084.233231
TCGGTAA40150.083.886551
AGGTCCG85050.083.144461
CGTTAGA35600.082.730811
GATCGTA31200.081.546771
TAGTACG24300.080.581517
CAATCGA22750.080.375768
TCGTAGG29200.080.125978
CACTACG28200.079.684712
GCGCAAC34050.079.4314961
CTGATAG39750.079.41211
CCGGGAT66550.079.200181
Produced by FastQC (version 0.11.5)