FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10600406
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2871142.708518900125146No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT408630.38548523518816163No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT203320.19180397430060697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT418650.0114.9563451
AGCAGTG455000.0105.8243262
GGTATCA479600.0100.362058
GTATCAA481000.0100.069929
TGGTATC490100.098.093917
CAGTGGT493150.097.422754
GTGGTAT494750.097.259716
AGTGGTA513150.094.083095
GCAGTGG573350.084.18743
AGAGTAC474850.026.00412815-19
CAACGCA482300.025.63255510-14
TCAACGC474000.025.24907310-14
AACGCAG501250.025.22651910-14
GTACTTT480750.024.10642620-24
CTACACG8250.023.0153731
GCAGAGT515300.022.94845215-19
CAGAGTA535500.022.70666315-19
ATCAACG465600.022.3740110-14
TACTTTT567250.021.24277920-24
CGCAGAG526300.019.80385615-19
Produced by FastQC (version 0.11.5)