FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10537698
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2835612.6909197815310324No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT405080.38441033326253987No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT200820.19057293158334962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT419800.0115.0974351
AGCAGTG453650.0106.5785452
GGTATCA472800.0102.2970058
GTATCAA476850.0101.152089
TGGTATC482500.099.929967
CAGTGGT487900.099.00294
GTGGTAT487150.098.886716
AGTGGTA505400.095.744415
GCAGTGG571500.084.722163
AGAGTAC468500.026.04045715-19
CAACGCA475850.025.47047610-14
TCAACGC466400.025.20244410-14
AACGCAG495150.025.1232110-14
GTACTTT474100.024.028920-24
GCAGAGT507350.022.9567615-19
CAGAGTA526800.022.65301915-19
ATCAACG459400.022.55895810-14
TACTTTT558800.021.17776720-24
CGCAGAG515150.020.07716415-19
ACGCAGA482450.019.88324415-19
Produced by FastQC (version 0.11.5)