FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004897988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450325
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT18920.420141009271082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.34996946649641925No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.31133070560151No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA13510.3000055515461056No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT10020.22250596791206353No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG8500.18875256759007383No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG7650.16987731083106644No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG7610.16898906345417197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.1623272081274635No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT7090.15744184755454393No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA6280.139454838172431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.13901071448398378No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT5460.12124576694609449No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA5190.11525009715205685No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.11302947870982069No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4540.10081607727752179No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)