FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8489282
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT287660.33885080033859166No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG150830.17767109161882005No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA140560.16557348430644664No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT134320.15822303935715648No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT88840.10464960405367615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87840.10347164813231556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA74500.053.9257285
TCAACGC75450.053.054334
AACGCAG77200.051.7599376
CGCAGAG83500.047.8544128
ATCAACG84650.047.543393
GGGGTTA105850.047.2048576
GGGTTAG106150.046.4610147
ACGCAGA86700.046.0053947
ATAGGGG102950.045.8757063
GAGTACG56500.045.152521
TAGGGGT105600.044.7244724
AGTACGG60500.042.3660352
GTATCAA104450.042.1370931
AGGGGTT109700.042.1353385
GGTTAGT130050.038.309928
GTACGGG74600.038.072331
GTTAGTC128200.036.3355529
GCGCGCT800.002108269635.9985929
TATAGGG145800.035.159742
GCAGAGT114500.035.149729
Produced by FastQC (version 0.11.5)