FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665437
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT55220.15065052270711513No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA45750.12481458554600722No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC44600.12167716973446821No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT39650.10817264080653959No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTA37230.10157042666399667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA221400.054.9869841
GGTATCA134750.046.0119671
TATCAAC285950.042.636722
TGATACG14600.042.3850172
ATGATAC14050.041.9267041
CAACGCA316650.038.146995
ATCAACG319050.038.138753
TCAACGC319300.037.9225354
ATATACG28350.037.7790761
TATACGA28350.037.7790762
AACGCAG323900.037.4681366
TACACAG17650.036.410185
TACGACA33700.033.3705834
ATACGAC35200.032.286623
ACGCAGA382100.031.6460937
CGCAGAG386600.031.2550898
ATGGGAG35900.030.1662065
ACGACAC36350.029.9564535
TCGTATA17500.029.81358130-31
ATGGGTG23550.029.5623325
Produced by FastQC (version 0.11.5)