FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359910
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.5119456434616997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48160.3541410828657779No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41940.30840276194748184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA1350.088.1307754
AAGTACG1400.080.808361
ATACGGG2750.077.9472051
GGTATCA30850.077.3962551
ATAACGC1700.069.988783
GTATCAA62000.059.254891
GTATAAC2850.041.784561
ATCAACG92750.041.5631453
TATCAAC93150.041.3238372
TCAACGC95500.040.613984
CAACGCA96800.040.135
AACGCAG100250.038.8083086
ACGCAGA111650.034.792517
CGCAGAG112100.034.6528478
GCAGAGT119100.032.6661039
GTCTCGC4600.032.360331
TCTGTCG4800.032.2228138
CGTTAGA750.003896928631.756271
TATCACG1501.479766E-731.7305772
AGAGTAC117250.030.36565410-11
Produced by FastQC (version 0.11.5)