FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004898019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821
Sequences flagged as poor quality0
Sequence length125
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA351.240694789081886TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.9571074087203121No Hit
GAAGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA170.6026231832683445TruSeq Adapter, Index 4 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.5671747607231479No Hit
GAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA150.5317263381779511RNA PCR Primer, Index 26 (96% over 31bp)
GATCGGAAGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC120.42538107054236085RNA PCR Primer, Index 11 (96% over 25bp)
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC120.42538107054236085No Hit
GATCGGAAGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC110.38993264799716415RNA PCR Primer, Index 46 (96% over 25bp)
GAAGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA100.3544842254519674TruSeq Adapter, Index 12 (96% over 27bp)
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA100.3544842254519674No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA90.31903580290677064No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT90.31903580290677064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.31903580290677064No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC80.28358738036157394No Hit
GATCGGAAGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC80.28358738036157394RNA PCR Primer, Index 26 (100% over 26bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC80.28358738036157394No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA70.24813895781637718No Hit
GAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAA70.24813895781637718RNA PCR Primer, Index 17 (96% over 27bp)
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC60.21269053527118043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.21269053527118043No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT50.1772421127259837No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT50.1772421127259837No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC50.1772421127259837No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC50.1772421127259837No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG50.1772421127259837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.1772421127259837No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT40.14179369018078697No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA40.14179369018078697TruSeq Adapter, Index 25 (96% over 27bp)
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT40.14179369018078697No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA40.14179369018078697No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC40.14179369018078697No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC40.14179369018078697No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG40.14179369018078697No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT40.14179369018078697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.14179369018078697No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC40.14179369018078697No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA40.14179369018078697No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC40.14179369018078697No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT40.14179369018078697No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG40.14179369018078697No Hit
GAAGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA40.14179369018078697Illumina PCR Primer Index 11 (96% over 29bp)
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC40.14179369018078697No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA30.10634526763559021TruSeq Adapter, Index 25 (96% over 27bp)
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT30.10634526763559021No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT30.10634526763559021No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT30.10634526763559021No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30.10634526763559021No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30.10634526763559021No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT30.10634526763559021No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA30.10634526763559021No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC30.10634526763559021No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG30.10634526763559021No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30.10634526763559021No Hit
GAGTAGCCGCGCTGGTGCCACACGACTACAACCCTCGTAATAGGGCTGCG30.10634526763559021No Hit
AAAGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA30.10634526763559021TruSeq Adapter, Index 11 (96% over 28bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT30.10634526763559021No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC30.10634526763559021No Hit
GAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAA30.10634526763559021TruSeq Adapter, Index 18 (96% over 27bp)
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG30.10634526763559021No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG30.10634526763559021No Hit
AGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCT30.10634526763559021No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC30.10634526763559021No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC30.10634526763559021No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGG30.10634526763559021No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC30.10634526763559021No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA30.10634526763559021No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT30.10634526763559021No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGG30.10634526763559021No Hit
GCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATA30.10634526763559021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)