FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011422
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA108360.27012864764664496No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC103880.25896053818321785No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC103210.25729030752685705No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA77150.19232581363915338No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC77020.19200173903418788No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT68970.17193404234209217No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC58740.1464318638128823No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG55370.13803085289954536No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA55050.13723313079501484No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA51740.12898169277627733No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC51430.1282088994875134No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC51340.1279845401456142No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA50350.12551658738472293No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT50060.12479365172749216No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT49960.12454436356982634No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC42900.10694461963862191No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC40880.10190899885377305No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG40460.10086198859157675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106500.071.613081
GGTATCA46900.065.199491
ATCAACG163250.046.2447433
TATCAAC170750.044.7709582
TCAACGC185150.040.9354634
CAACGCA188900.039.9653475
TCTCTGC18650.037.959648
TTCTCTG26650.035.7171447
ACTGTGC34900.033.9220128
CTGTGCA32650.033.5265279
CACTGTG32200.033.44087
CGCAGAG231750.032.4731758
ACGCAGA233400.032.294597
GCAGAGT235300.032.0338139
AACGCAG242450.031.7025536
ATTTAGG54050.027.8470251
CTATGCA1306.3884734E-527.4575564
TATTCTC30050.026.7265725
TATAATA18750.026.6521342
ATAATAC18800.025.6319183
Produced by FastQC (version 0.11.5)