FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078823
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117340.38111966813291964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73080.23736343401358248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56960.185005763566142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.14041079984136795No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA36880.11978603511796553No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC34050.11059421083966178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201550.072.3026661
GGTATCA118500.057.2493551
TATCAAC300600.048.4043462
ATCAACG314300.046.248643
CAACGCA315800.046.1414575
TCAACGC323800.044.867324
AACGCAG353500.041.3420946
ACGCAGA358650.040.8702137
CGCAGAG361800.040.5342758
GCAGAGT373400.039.2557649
ATTTAGG19500.035.0886231
CTATTGA21300.031.4342659
TTTAGGG25400.028.3447952
GGCTATT25850.028.129427
GGGCTAT28700.026.589486
TACTGTG12700.026.0767867
TATGGTG18550.025.2264024
GCTATTG26900.025.1579698
TAAGACT12400.024.9658729
GTATAAG19050.024.4544581
Produced by FastQC (version 0.11.5)