FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5877819
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA247450.063.1005741
GGTATCA123800.048.210011
CAACGCA333500.046.3431365
ATCAACG337900.045.6950383
TCAACGC338850.045.6184434
AACGCAG341650.045.3676456
TATCAAC343700.045.0812722
ACGCAGA352500.043.8760037
CGCAGAG364850.042.389028
GCAGAGT384000.040.0304079
AGAGTAC367750.031.69386110-11
CAGAGTA376300.028.26353610-11
AGTACGG426400.027.1824512-13
TACGGGT75800.025.61896914-15
GAGTACG430250.024.56872712-13
TACGGGG134000.023.7685314-15
GTACGGG436650.023.58504514-15
TACGGGC116150.023.14551714-15
TACGGGA161950.021.75980814-15
ACGGGCT50600.017.98792816-17
Produced by FastQC (version 0.11.5)