FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004898040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004898040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9238556
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG150710.16313155432515644No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA113550.12290881821791198No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC106570.1153535249448074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA75400.048.4756935
CGCAGAG82600.044.5810628
ATCAACG82500.044.231693
TCAACGC83500.043.630684
TCTGTCG13000.043.4730458
ACGCAGA85650.042.993067
GGTATCA37150.040.072131
GTATCAA97100.039.7390061
AACGCAG94500.038.8401956
GCAGAGT100250.036.4368069
GCAGCGC9900.036.0542038
GTCTCGC12500.032.870051
CTCGCTC12050.030.1349013
TCTCGCT13750.029.4396882
TGGTCCT36800.027.9849995
ACGGGAC11300.026.340173
AATGCGT41650.025.4233676
GCTCTAG20150.025.119161
GTACGGG123550.025.0623531
GAGTACG96050.024.81487512-13
Produced by FastQC (version 0.11.5)